Journal: Journal of Dental Sciences

Article Title: Kartogenin potentially protects temporomandibular joints from collagenase-induced osteoarthritis via core binding factor β and runt-related transcription factor 1 binding - A rat model study

doi: 10.1016/j.jds.2023.03.002

Figure Lengend Snippet: Kartogenin promoted the cell proliferation and matrix formation, and reduced cell apoptosis and local inflammation in TMJOA. (A) The expression of PCNA, CASP3, IL-1β, and COL2 was detected by immunohistochemistry in the TMJ condylar cartilage in the dimethylsulfoxide, kartogenin, Ro5-3335 (specific inhibitor of CBFβ-RUNX1 binding), kartogenin + Ro5-3335 groups. Scale bar: 100 μm. (B–E) Quantitative analysis of ratio of PCNA + , CASP3 + , IL-1β + , COL2 + cells in TMJ condylar cartilage at 4 weeks. ∗ P < .05, ∗∗ P < .01, ∗∗∗ P < .001. Abbreviations: KGN, kartogenin; DMSO, dimethylsulfoxide; R5, Ro5-3335.

Article Snippet: Diluted primary antibodies against Proliferating Cell Nuclear Antigen (PCNA) (10205-2-AP, Proteintech, Rosemont, IL, USA), Caspase-3 (CASP3) (19677-1-AP, Proteintech), Interleukin-1 beta (IL-1β) (ab254360, Abcam, Waltham, MA, USA), and Collagen II (COL2) (BA0533, Boster, Pleasanton, CA, USA) were added and incubated with the sections at 4 °C overnight.

Techniques: Expressing, Immunohistochemistry, Binding Assay